The Joint Annual Scientific Meetings of the Endocrine Society of Australia and the Society for Reproductive Biology 2018

Exposure to bisphenol A and 4-tert-octylphenol result in embryo implantation failure in mouse (#387)

Dinh Nam Tran 1 , Eui-Man Jung 1 , Jae-Hwan Lee 1 , Seon Young Park 1 , Bo-Hui Jeon 1 , Duc Viet Ly 1 , Thi Hoai Thu Nguyen 1 , Eui-Bae Jeung 1
  1. Chungbuk National University, Seowon-gu, Chungbuk, South Korea

In human, miscarriage due to blastocyst implantation failure is up to 2/3 of all cases. Calcium (Ca2+) has been showed to involve in many cellular signal transduction pathways as well as regulation of cell adhesion, which is necessary for the process of embryo implantation. Previous studies have been reported that EDs can regulate the expression of genes associated with calcium transport in during pregnancy period such as TRPV5, TRPV6, PMCA, and NCX1. Additionally, exposure to EDs during early gestation results in disrupt intrauterine implantation, uterine receptive, leading to implantation failure. In this study, E2, BPA, OP and/or ICI 182,780 were inject by subcutaneous from gestation day 1 to gestation day 3 post coitus. The expression levels of calcium transport genes were assessed in maternal uterus and implantation site. The number of implantation sites were significantly decreased in OP group and no implantation site was observed in EDs+ICI groups. There was different in the expression of calcium transient transport channel between maternal uterus and implantation site. The levels of TRPV6 and TRPV5 genes were significantly increased by EDs and/or ICI treatment in uterus. The NCX1 and PMCA1 mRNA levels were significantly decreased in OP and BPA groups, but no significantly different in protein level in uterus. In contrary, NCX1 and PMCA1 mRNA levels were significantly decreased by OP- and BPA- treated in implantation site. Both mRNA and protein levels of MUC1 markedly higher in all groups, except BPA-group when compared to the VE in uterus. The LIF and HOXA-10 mRNA were significantly decreased by E2; BPA+ICI; OP and/or ICI in both uterus and implantation site. The expression of estrogen receptor (ERĪ±) and progesterone receptor (PR) were significantly lower in all groups. Taken together, these results suggest that E2, BPA and OP impairs embryo implantation through altered expression of calcium transport genes.